The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 20, 2015
Filed:
Dec. 19, 2008
John S. Haurum, København, DK;
Finn C. Wiberg, Farum, DK;
Vincent W. Coljee, Hellerup, DK;
Jacqueline Sharon, Chestnut Hill, MA (US);
Chiou-ying Yang, Taichung, TW;
John S. Haurum, København, DK;
Finn C. Wiberg, Farum, DK;
Vincent W. Coljee, Hellerup, DK;
Jacqueline Sharon, Chestnut Hill, MA (US);
Chiou-Ying Yang, Taichung, TW;
Symphogen A/S, Lyngby, DK;
Abstract
The invention relates to a method for manufacturing a recombinant polyclonal protein composition, in particular a recombinant polyclonal antibody composition. The method comprises obtaining a collection of cells transfected with a library of variant nucleic acid sequences, wherein each call in the collection is transfected with and capable of expressing one member of the library, which encodes a distinct member of a polyclonal protein that binds a particular antigen and which is located at the same single site in the genome of individual cells in said collection, wherein said nucleic acid sequence is not naturally associated with said cell in the collection. The cells are cultured under suitable conditions for expression of the polyclonal protein, which is obtained from the cells or culture supernatant. The present method is suitable for manufacturing recombinant polyclonal antibodies, thereby making available a superior replacement of plasma-derived therapeutic immunoglobulin products.