The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 13, 2015

Filed:

Aug. 07, 2013
Applicant:

Sony Corporation, Minato-ku, JP;

Inventors:

Michael Hulko, Stuttgart, DE;

Nadejda Krasteva, Stuttgart, DE;

Ingeborg Hospach, Stuttgart, DE;

Gabriele Nelles, Stuttgart, DE;

Assignee:

SONY Corporation, Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/02 (2006.01); C07H 21/04 (2006.01); A61K 48/00 (2006.01); C12Q 1/68 (2006.01); G01N 33/53 (2006.01); G01N 33/542 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6834 (2013.01); G01N 33/5308 (2013.01); G01N 33/542 (2013.01); G01N 2333/9005 (2013.01);
Abstract

The present invention relates to aptazyme sensor devices comprising, in addition to the aptamer, ribozyme and communication components, a competitive inhibitory component with a metal nanoparticle or a competitive inhibitory and signalling component with a metal nanoparticle and a label, such that the enzymatic activity of the ribozyme is inhibited as long as the inhibitory or the inhibitory and signalling component is bound to the substrate binding site of the ribozyme. The aptazyme sensor devices of the invention have increased shelf-life and are suitable for the parallel detection of different ligands by using an array of aptazyme sensor devices utilizing inhibitory and inhibitory and signalling components with different metal nanoparticles. The present invention furthermore relates to the post-synthetic chemical modification of the aptamer component for avoiding unspecific binding.


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