The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 06, 2015
Filed:
Mar. 28, 2013
Genewiz Inc., Suzhou, Suzhou, Jiangsu, CN;
Shihong Li, Berkeley Heights, NJ (US);
Narisra Jongkam, Highland Park, NJ (US);
Shifang Zhang, New York, NY (US);
Conrad Leung, Edison, NJ (US);
Genewiz Inc., Suzhou, Suzhou, Jiangsu, CN;
Abstract
This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2'-deoxyinosine-5′ triphosphate, 5-propynyl-2′-deoxycytidine-5′-triphosphate, and 8-oxo-2′-deoxyguanosine-5′-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures.