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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 9150891 B1

Date of Patent:

Oct. 06, 2015

Filed:

Sep. 24, 2010

Method for transforming stramenopiles

Applicants:

Keishi Sakaguchi, Fukuoka, JP;

Takanori Matsuda, Fukuoka, JP;

Takumi Kobayashi, Fukuoka, JP;

Makoto Ito, Fukuoka, JP;

Naoki Nagano, Miyazaki, JP;

Masahiro Hayashi, Miyazaki, JP;

Daisuke Honda, Kobe, JP;

Yosuke Taoka, Chiyoda-ku, JP;

Yuji Okita, Chiyoda-ku, JP;

Hitoshi Izumida, Chiyoda-ku, JP;

Shinichi Sugimoto, Chiyoda-ku, JP;

Inventors:

Keishi Sakaguchi, Fukuoka, JP;

Takanori Matsuda, Fukuoka, JP;

Takumi Kobayashi, Fukuoka, JP;

Makoto Ito, Fukuoka, JP;

Naoki Nagano, Miyazaki, JP;

Masahiro Hayashi, Miyazaki, JP;

Daisuke Honda, Kobe, JP;

Yosuke Taoka, Chiyoda-ku, JP;

Yuji Okita, Chiyoda-ku, JP;

Hitoshi Izumida, Chiyoda-ku, JP;

Shinichi Sugimoto, Chiyoda-ku, JP;

Assignees:

KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION, Fukuoka, JP;

UNIVERSITY OF MIYAZAKI, Miyazaki, JP;

KONAN GAKUEN, Hyogo, JP;

NIPPON SUISAN KAISHA, LTD., Tokyo, JP;

Attorney:

Westerman, Hattori, Daniels & Adrian, LLP

Primary Examiner:

Iqbal H Chowdhury

Int. Cl.

CPC ...

C12P 7/64 (2006.01); C12P 21/06 (2006.01); C12N 1/00 (2006.01); C12N 1/10 (2006.01); C12N 15/00 (2006.01); C11C 3/00 (2006.01); C12N 9/02 (2006.01); C12N 15/79 (2006.01); C12N 15/82 (2006.01);

U.S. Cl.

CPC ...

C12P 7/6409 (2013.01); C11C 3/00 (2013.01); C12N 9/0083 (2013.01); C12N 15/79 (2013.01); C12N 15/8201 (2013.01); C12N 15/8247 (2013.01); C12P 7/6427 (2013.01);

Abstract

Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class, more specifically, to a genus, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (Δ5 desaturase gene, Δ12 desaturase gene and/or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.

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