The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 06, 2015
Filed:
Jul. 08, 2013
Applicant:
Chang Gung University, Tao-Yuan, TW;
Inventors:
Hsiu-Chuan Yen, Tao-Yuan, TW;
Shih-Wei Chen, Taipei, TW;
Assignee:
Chang Gung University, Tao-Yuan, TW;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/10 (2006.01);
U.S. Cl.
CPC ...
C12N 9/1007 (2013.01); C12Y 201/01201 (2013.01);
Abstract
The present invention relates to a method of producing and purifying soluble recombinant coenzyme Q5 homolog (COQ5) protein, which is expressed in soluble form by(), under native conditions. The method is characterized by pre-treating bacterial lysate with low concentration of ionic detergent, such as sodium dodecyl sulfate (SDS), before purification; and the purifying method is performed under native condition without using urea to avoid the problems of requiring lengthy processes to remove urea in purified protein solution or re-aggregation and precipitation of protein after removal of urea.