The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 06, 2015

Filed:

Jul. 09, 2010
Applicants:

Hilde Stals, Ghent, BE;

Veronique DE Brabandere, Ghent, BE;

Peter Schotte, De Pinte, BE;

Inventors:

Hilde Stals, Ghent, BE;

Veronique De Brabandere, Ghent, BE;

Peter Schotte, De Pinte, BE;

Assignee:

Ablynx N.V., Zwijnaarde, BE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/04 (2006.01); C12N 5/00 (2006.01); C12N 5/07 (2010.01); C12N 5/16 (2006.01); C12N 1/12 (2006.01); C07H 21/04 (2006.01); A61K 47/48 (2006.01); C07K 16/00 (2006.01);
U.S. Cl.
CPC ...
C07K 16/00 (2013.01); A61K 47/48215 (2013.01); A61K 47/48338 (2013.01); C07K 2317/569 (2013.01); C07K 2319/00 (2013.01);
Abstract

The present invention provides methods for the expression and/or production of variable domains with a C-terminal extension that can be used for coupling of the variable domain to one or more further groups, residues or moieties. In the method of the invention a yield of at least 80% of variable domains with a cysteine containing C-terminal extension is obtained. Also variable domains are provided and polypeptides comprising one or more variable domains obtainable by the methods of the present invention, as well as compounds that comprise such variable domains and/or polypeptides coupled to one or more groups, residues or moieties.


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