The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 06, 2015

Filed:

May. 16, 2011
Applicant:

Robert J. Hariri, Florham Park, NJ (US);

Inventor:

Robert J. Hariri, Florham Park, NJ (US);

Assignee:

Anthrogenesis Corporation, Warren, NJ (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/071 (2010.01); A61M 1/02 (2006.01); C12N 5/073 (2010.01); C12N 5/0775 (2010.01); A61K 35/12 (2015.01); A61K 48/00 (2006.01);
U.S. Cl.
CPC ...
A61M 1/02 (2013.01); C12N 5/0603 (2013.01); C12N 5/0605 (2013.01); C12N 5/0668 (2013.01); A61K 35/12 (2013.01); A61K 48/00 (2013.01); A61M 2202/0462 (2013.01); A61M 2210/1458 (2013.01); A61M 2210/1466 (2013.01); C12N 2509/00 (2013.01); C12N 2510/02 (2013.01); C12N 2533/90 (2013.01);
Abstract

The present invention provides a method of extracting and recovering embryonic-like stem cells, including, but not limited to pluripotent or multipotent stem cells, from an exsanguinated human placenta. A placenta is treated to remove residual umbilical cord blood by perfusing an exsanguinated placenta, preferably with an anticoagulant solution, to flush out residual cells. The residual cells and perfusion liquid from the exsanguinated placenta are collected, and the embryonic-like stem cells are separated from the residual cells and perfusion liquid. The invention also provides a method of utilizing the isolated and perfused placenta as a bioreactor in which to propagate endogenous cells, including, but not limited to, embryonic-like stem cells. The invention also provides methods for propagation of exogenous cells in a placental bioreactor and collecting the propagated exogenous cells and bioactive molecules therefrom.


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