The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 29, 2015

Filed:

Jan. 26, 2015
Applicant:

National Medical Services, Inc., Willow Grove, PA (US);

Inventors:

Bingfang Yue, North Wales, PA (US);

Shuguang Li, Abington, PA (US);

Wenzhe Li, Middletown, DE (US);

Assignee:

Other;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/58 (2006.01); G01N 33/567 (2006.01); G01N 30/72 (2006.01); H01J 49/00 (2006.01);
U.S. Cl.
CPC ...
G01N 30/7233 (2013.01); H01J 49/004 (2013.01);
Abstract

The present invention provides methods for determining the amount of cis-diol containing compounds in a biological samples using mass spectrometry, particularly catecholamines (dopamine, epinephrine, norepinephrine) in a plasma sample. After a sample is mixed with an internal standard, extracted with a two-step liquid-liquid extraction process utilizing phenylboronic acid chemistry, and then derivatized with a benzoyl chloride-like agent, relevant moieties are then separated chromatographically and detected by MS/MS whereby specific ion transitions are monitored. This invention has several clinical utilities, particularly those related to measuring plasma catecholamines in patients.


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