The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 22, 2015

Filed:

May. 09, 2012
Applicants:

Carrie Graham, Raleigh, NC (US);

Allen Eckhardt, San Diego, CA (US);

Lisa Perkins, Madison, WI (US);

Inventors:

Carrie Graham, Raleigh, NC (US);

Allen Eckhardt, San Diego, CA (US);

Lisa Perkins, Madison, WI (US);

Assignee:

Advanced Liquid Logic, Inc., San Diego, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/573 (2006.01); G01N 1/40 (2006.01); C12N 9/16 (2006.01); C12N 9/24 (2006.01); C12N 9/26 (2006.01); C12N 9/38 (2006.01); G06F 19/10 (2011.01);
U.S. Cl.
CPC ...
G01N 1/405 (2013.01); C12N 9/16 (2013.01); C12N 9/2402 (2013.01); C12N 9/2408 (2013.01); C12N 9/2471 (2013.01); C12Y 301/06013 (2013.01); C12Y 302/01023 (2013.01); C12Y 302/01076 (2013.01); G01N 33/573 (2013.01); G01N 2333/924 (2013.01); G01N 2333/928 (2013.01); G01N 2333/938 (2013.01); G01N 2800/38 (2013.01); G06F 19/10 (2013.01);
Abstract

Methods of conducting an assay for measuring enzymatic modification of a substrate by a glycoprotein having enzymatic activity are provided. The methods include concentrating the glycoprotein having enzymatic activity by capturing the glycoproteins from the input sample on a solid support, washing the glycoproteins captured on a solid support to remove unbound portions of the input sample, adding an enzyme substrate to the output sample comprising glycoproteins having enzymatic activity, and measuring enzymatic modification of the substrate. In some examples, the capturing of the glycoprotein and subsequent washing steps are performed in one or more droplets in oil.


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