The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 22, 2015
Filed:
Jul. 06, 2012
Jason Myers, Menlo Park, CA (US);
Zhoutao Chen, Carlsbad, CA (US);
Devin Dressman, Swampscott, MA (US);
Theo Nikiforov, Carlsbad, CA (US);
Jason Myers, Menlo Park, CA (US);
Zhoutao Chen, Carlsbad, CA (US);
Devin Dressman, Swampscott, MA (US);
Theo Nikiforov, Carlsbad, CA (US);
Life Technologies Corporation, Carlsbad, CA (US);
Abstract
In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template. In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.