The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 22, 2015

Filed:

Aug. 03, 2010
Applicants:

Hiroshi Handa, Yokohama, JP;

Akira Nakanishi, Yokohama, JP;

Masaaki Kawano, Yokohama, JP;

Inventors:

Hiroshi Handa, Yokohama, JP;

Akira Nakanishi, Yokohama, JP;

Masaaki Kawano, Yokohama, JP;

Assignee:

TOKYO INSTITUTE OF TECHNOLOGY, Meguro-ku, Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/70 (2006.01); A61K 6/00 (2006.01); A61K 39/12 (2006.01); C12N 7/00 (2006.01); C07K 14/005 (2006.01); A61K 39/00 (2006.01);
U.S. Cl.
CPC ...
C12N 7/00 (2013.01); A61K 39/12 (2013.01); C07K 14/005 (2013.01); A61K 2039/5258 (2013.01); C12N 2710/22022 (2013.01); C12N 2710/22023 (2013.01);
Abstract

There is provided a novel method of forming uniform viral particles under physiological conditions. The method of forming uniform-sized viral particle aggregates composed of viral protein is characterized by incubating a viral protein such as SV40 virus VP1 at pH 5.0 to 7.0, room temperature, in the presence of 130 mM to 170 mM sodium chloride and 1.5 mM to 2.5 mM divalent cation, and in the presence of a particle formation acceleration factor such as SV40 VP2. For encapsulation of a substance to be encapsulated in the viral particles, the substance to be encapsulated is included during the incubation.


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