IDiyas

For the Inventor, By the Inventor

The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 9139632 B1

PDFFull Text
Date of Patent:
Sep. 22, 2015

Filed:

Oct. 11, 2010

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Applicants:

Piotr Bobrowicz, Hanover, NH (US);

Sujatha Gomathinayagam, Hanover, NH (US);

Stephen Hamilton, Enfield, NH (US);

Huijuan LI, Hanover, NH (US);

Natarajan Sethuraman, Hanover, NH (US);

Terrance A. Stadheim, Lyme, NH (US);

Stefan Wildt, New York, NY (US);

Inventors:

Piotr Bobrowicz, Hanover, NH (US);

Sujatha Gomathinayagam, Hanover, NH (US);

Stephen Hamilton, Enfield, NH (US);

Huijuan Li, Hanover, NH (US);

Natarajan Sethuraman, Hanover, NH (US);

Terrance A. Stadheim, Lyme, NH (US);

Stefan Wildt, New York, NY (US);

Assignee:

Merck Sharp & Dohme Corp., Rahway, NJ (US);

Attorney:
Primary Examiner:

Shulamith H Shafer

Int. Cl.
CPC ...
C12P 21/06 (2006.01); C12N 1/00 (2006.01); C12N 5/00 (2006.01); C12P 21/04 (2006.01); C07K 1/00 (2006.01); C07K 14/505 (2006.01); C07K 14/47 (2006.01); C07K 14/485 (2006.01); C07K 14/52 (2006.01); C07K 14/535 (2006.01); C07K 14/54 (2006.01); C07K 14/555 (2006.01); C07K 14/59 (2006.01); C07K 14/605 (2006.01); C07K 14/705 (2006.01); C07K 14/715 (2006.01); C07K 14/755 (2006.01); C07K 14/81 (2006.01); C07K 16/18 (2006.01); C07K 16/22 (2006.01); C12N 9/10 (2006.01); C12P 21/00 (2006.01);
U.S. Cl.
CPC ...
C07K 14/505 (2013.01); C07K 14/47 (2013.01); C07K 14/4715 (2013.01); C07K 14/4721 (2013.01); C07K 14/4743 (2013.01); C07K 14/485 (2013.01); C07K 14/52 (2013.01); C07K 14/523 (2013.01); C07K 14/535 (2013.01); C07K 14/54 (2013.01); C07K 14/555 (2013.01); C07K 14/59 (2013.01); C07K 14/605 (2013.01); C07K 14/705 (2013.01); C07K 14/70546 (2013.01); C07K 14/70578 (2013.01); C07K 14/7151 (2013.01); C07K 14/755 (2013.01); C07K 14/8114 (2013.01); C07K 14/8125 (2013.01); C07K 14/8128 (2013.01); C07K 16/18 (2013.01); C07K 16/22 (2013.01); C12N 9/1051 (2013.01); C12P 21/005 (2013.01); C07K 2317/33 (2013.01);
Abstract

Methods for producing proteins and glycoproteins inthat lack detectable cross binding activity to antibodies made against host cell antigens are described. In particular, methods are described wherein recombinantstrains that do not display a β-mannosyltransferase 2 activity with respect to an N-glycan or O-glycan and do not display at least one activity selected from a β-mannosyltransferase 1, 3, and 4 activity to produce recombinant proteins and glycoproteins. These recombinantstrains can produce proteins and glycoproteins that lack detectable α-mannosidase resistant β-mannose residues thereon and thus, lack cross binding activity to antibodies against host cell antigens. Further described are methods for producing bi-sialylated human erythropoietin inthat lack detectable cross binding activity to antibodies against host cell antigens.

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