The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 15, 2015
Filed:
Jun. 29, 2007
Caixia LI, Beijing, CN;
Huafang Gao, Beijing, CN;
Xiang Liu, Beijing, CN;
Bin Cai, Beijing, CN;
Di Zhang, Beijing, CN;
Jing Cheng, Beijing, CN;
Caixia Li, Beijing, CN;
Huafang Gao, Beijing, CN;
Xiang Liu, Beijing, CN;
Bin Cai, Beijing, CN;
Di Zhang, Beijing, CN;
Jing Cheng, Beijing, CN;
CapitalBio Corporation, Beijing, CN;
Tsinghua University, Beijing, CN;
Abstract
The invention provides a method for identification of alleles. In this method, genomic DNA is used as target. Multiple allele-specific PCR amplification are carried out with a group of primers comprising one or more allele-specific primers for a target gene, a universal primer, and a common primer; and a DNA polymerase without 5' to 3′ exonuclease activity. The PCR products are hybridized with tag probes immobilized on a DNA chip. Results are determined based on the signal intensity and the position of the probe immobilized on the array. Each allele-specific primer comprises a unique tag sequence at the 5′ end. Each tag probe immobilized on the DNA chip comprises a sequence identical to its corresponding tag sequence; and each tag probe hybridizes only with the complementary sequence in the PCR amplification product.