The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 15, 2015

Filed:

Sep. 12, 2011
Applicants:

Dino Di Carlo, Los Angeles, CA (US);

Soojung C. Hur, Boston, MA (US);

Albert J. Mach, San Jose, CA (US);

Inventors:

Dino Di Carlo, Los Angeles, CA (US);

Soojung C. Hur, Boston, MA (US);

Albert J. Mach, San Jose, CA (US);

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12M 1/00 (2006.01); C12Q 1/24 (2006.01); B01L 3/00 (2006.01); C12M 3/06 (2006.01); C12Q 1/68 (2006.01); G01N 33/58 (2006.01); G01N 1/40 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/24 (2013.01); B01L 3/502746 (2013.01); B01L 3/502761 (2013.01); C12M 23/16 (2013.01); C12M 29/00 (2013.01); C12M 47/04 (2013.01); C12Q 1/68 (2013.01); G01N 33/582 (2013.01); B01L 2200/0668 (2013.01); B01L 2300/087 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/082 (2013.01); B01L 2400/086 (2013.01); G01N 1/40 (2013.01);
Abstract

A method of isolating cells includes providing a microfluidic device having at least one microfluidic channel coupled to an inlet and an outlet, the at least one microfluidic channel comprises at least one expansion region disposed along the length thereof. The at least one expansion region is an abrupt increase in a cross-sectional dimension of the at least one microfluidic channel configured to generate a vortex within the at least one expansion region in response to fluid flow. A solution containing a population of cells at least some of which have diameters ≧10 μm flows into the inlet. A portion of cells is trapped within vortex created within the at least one expansion region. The trapped cells may then released from the expansion region.


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