The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 15, 2015

Filed:

Jul. 05, 2007
Applicants:

Gottfried Himmler, Gross-Enzersdorf, AT;

Gerda Redl, Gross-Enzersdorf, AT;

Florian Ruker, Vienna, AT;

Gordana Wozniak-knopp, Vienna, AT;

Inventors:

Gottfried Himmler, Gross-Enzersdorf, AT;

Gerda Redl, Gross-Enzersdorf, AT;

Florian Ruker, Vienna, AT;

Gordana Wozniak-Knopp, Vienna, AT;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 16/24 (2006.01); C07K 16/10 (2006.01); C07K 16/16 (2006.01); C07K 16/32 (2006.01); C07K 16/40 (2006.01);
U.S. Cl.
CPC ...
C07K 16/241 (2013.01); C07K 16/1063 (2013.01); C07K 16/16 (2013.01); C07K 16/32 (2013.01); C07K 16/40 (2013.01); C07K 2317/21 (2013.01); C07K 2317/22 (2013.01); C07K 2317/55 (2013.01); C07K 2317/622 (2013.01);
Abstract

A method for engineering an immunoglobulin having a variable domain and at least one modification in at least two structural loops of the immunoglobulin and determining the binding of the immunoglobulin to an epitope of an antigen, where the unmodified immunoglobulin does not significantly bind to said epitope, comprising the steps of providing a nucleic acid encoding an immunoglobulin having at least two structural loops, modifying at least one nucleotide residue of each of the structural loops, transferring the modified nucleic acid in an expression system, expressing the modified immunoglobulin, contacting the expressed modified immunoglobulin with an epitope, and determining whether the modified immunoglobulin binds to the epitope.


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