The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 08, 2015

Filed:

May. 04, 2012
Applicants:

Pei-jer Chen, Taipei, TW;

Ping-hei Chen, Taipei, TW;

Wen-pin Chou, Taipei, TW;

Yi-fan Hsieh, Banciao, TW;

Shiou-hwei Yeh, Sanchong, TW;

Inventors:

Pei-Jer Chen, Taipei, TW;

Ping-Hei Chen, Taipei, TW;

Wen-Pin Chou, Taipei, TW;

Yi-Fan Hsieh, Banciao, TW;

Shiou-Hwei Yeh, Sanchong, TW;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); B01L 7/00 (2006.01); B01L 7/02 (2006.01);
U.S. Cl.
CPC ...
B01L 7/525 (2013.01); C12Q 1/686 (2013.01); B01L 7/02 (2013.01); B01L 7/54 (2013.01); B01L 2300/0838 (2013.01); B01L 2300/1838 (2013.01); B01L 2400/0472 (2013.01);
Abstract

The present invention provides a method and apparatus for amplifying a nucleic acid sequence by polymerase chain reaction (PCR). The method comprises placing a PCR sample in a container which is heated by only a single heat source that provides a high temperature for denaturation in the bottom of the PCR sample, while annealing and extension automatically occur in different regions of the PCR sample due to the convection induced by a temperature gradient descending from the bottom of the PCR sample to the surface of the PCR sample.


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