The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 01, 2015

Filed:

Jul. 21, 2011
Applicants:

Kenji Osafune, Kyoto, JP;

Shin-ichi Mae, Kyoto, JP;

Masatoshi Kajiwara, Kyoto, JP;

Inventors:

Kenji Osafune, Kyoto, JP;

Shin-ichi Mae, Kyoto, JP;

Masatoshi Kajiwara, Kyoto, JP;

Assignee:

KYOTO UNIVERSITY, Kyoto-shi, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/071 (2010.01); C12N 5/074 (2010.01); C12N 5/073 (2010.01); C12N 5/0735 (2010.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0696 (2013.01); C12N 5/0603 (2013.01); C12N 5/0606 (2013.01); G01N 33/5073 (2013.01); C12N 2501/155 (2013.01); C12N 2501/16 (2013.01); C12N 2501/415 (2013.01); C12N 2506/45 (2013.01);
Abstract

The present invention relates to: a method for producing an intermediate mesoderm cell from a human pluripotent stem cell, comprising a step of culturing the human pluripotent stem cell in a medium containing Activin A and Wnt or a functional equivalent of Wnt and a step of culturing cells in a medium containing BMP and Wnt or a functional equivalent of Wnt; to a method for producing a metanephric cell from the intermediate mesoderm cell produced by the first method; to a human pluripotent stem cell having a foreign reporter gene in the chromosome wherein the gene is expressed interlocked with the expression of endogenous OSR1; to a method for screening for an inducer for differentiation into intermediate mesoderm using the human pluripotent stem cell; and to a kit for inducing the differentiation into an intermediate mesoderm cell.


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