The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 25, 2015
Filed:
Oct. 23, 2008
Applicant:
Weidong Cao, Rockville, MD (US);
Inventor:
Weidong Cao, Rockville, MD (US);
Assignee:
Canon U.S. Life Sciences, Inc., Rockville, MD (US);
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); B01L 9/00 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502784 (2013.01); B01L 7/52 (2013.01); B01L 9/52 (2013.01); B01L 2300/0867 (2013.01);
Abstract
The present invention relates to a method for reducing cross-contamination in continuous amplification reactions in channels of microfluidic devices. More specifically, the present invention relates to the use of specific materials continuously flowing in the channels to reduce adsorption of MgCland the concomitant adsorption of nucleic acid template to the channel surface, thereby reducing cross-contamination. This reduction of cross-contamination improves the efficiency and reproducibility of the amplification reaction, e.g., PCR.