Cultured periodontal ligament cell sheet, process for producing the same and method of use thereof

Abstract

A cultured periodontal membrane cell sheet, produced through a process comprising culturing cells on a cell culture support composed of a base material having its surface coated with a temperature-responsive polymer of 0˚C to 80˚C upper limit or lower limit critical dissolution temperature in water under specified conditions, (1) regulating the temperature of culture fluid to temperature≧the upper limit critical dissolution temperature or≦the lower limit critical dissolution temperature, (2) causing a cultured periodontal membrane cell sheet resulting from the culturing to adhere to a carrier, and (3) detaching the sheet together with the carrier. This cultured periodontal membrane cell sheet has a syndesmotic microstructure, exhibiting extremely high bioadherence to the surface of dental root, and realizes high-density transplant of target cells and positive reconstruction of periodontal tissue. Moreover, through laminating of cell sheets to be transplanted and donating of three-dimensional polarity, highly efficient reconstruction of attachment apparatus can be accomplished, and clinical application thereof to moderate periodontitis, severe periodontitis, gingival recession, etc. is highly promising.