The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 18, 2015

Filed:

Dec. 27, 2011
Applicants:

Mark Eshoo, Solana Beach, CA (US);

John Picuri, Carlsbad, CA (US);

Stanley Motley, Carlsbad, CA (US);

Inventors:

Mark Eshoo, Solana Beach, CA (US);

John Picuri, Carlsbad, CA (US);

Stanley Motley, Carlsbad, CA (US);

Assignee:

IBIS BIOSCIENCES, INC., Carlsbad, CA (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C40B 50/06 (2006.01); C12N 9/12 (2006.01); C09K 3/00 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2006.01);
U.S. Cl.
CPC ...
C12N 15/10 (2013.01); C12N 15/1093 (2013.01); C12Q 1/686 (2013.01);
Abstract

The present invention provides compositions and methods for preparing a nucleic acid library in a multi-purpose buffer (e.g., employing whole genome amplification), where nucleic acid purification is not required between or during steps. In certain embodiments, small amounts of starting nucleic acid (e.g., genomic DNA) are employed and the steps are accomplished in a single container. In some embodiments, the nucleic acid library is subjected to sequencing methodologies or rolling circle amplification.


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