The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 18, 2015
Filed:
Jul. 04, 2013
Applicant:
Jiangnan University, Wuxi, CN;
Inventors:
Assignee:
Jiangnan University, Wuxi, JS, CN;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/00 (2006.01); C12N 9/18 (2006.01); C12N 9/20 (2006.01); C12P 21/00 (2006.01); C12P 21/02 (2006.01); C12N 9/26 (2006.01); C12N 9/24 (2006.01); C12N 9/42 (2006.01);
U.S. Cl.
CPC ...
C12N 9/20 (2013.01); C12N 9/2408 (2013.01); C12N 9/248 (2013.01); C12N 9/2414 (2013.01); C12N 9/2434 (2013.01); C12P 21/00 (2013.01); C12P 21/02 (2013.01);
Abstract
The present invention provides a method of increasing extracellular secretion of secretory proteins by co-expressing the secretory proteins with a mature cutinase. Cutinase can improve the permeability ofcell membrane without destroying the membrane, and thus facilitate cross-membrane transfer of the secretory proteins co-expressed in. Increased extracellular secretion of target proteins can shorten cell culture time, reduce the formation of inclusion bodies and increase production of target proteins.