The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 18, 2015

Filed:

Apr. 29, 2011
Applicants:

Zaeem Ashraf Khan, Albany, NY (US);

Aaron Joseph Dulgar-tulloch, Ballston spa, NY (US);

Stefan Rakuff, Clifton Park, NY (US);

Philip Alexander Shoemaker, Scotia, NY (US);

Erik Leeming Kvam, Albany, NY (US);

Xiaohui Chen, Niskayuna, NY (US);

Jaydeep Roy, Saratoga Springs, NY (US);

Inventors:

Zaeem Ashraf Khan, Albany, NY (US);

Aaron Joseph Dulgar-Tulloch, Ballston spa, NY (US);

Stefan Rakuff, Clifton Park, NY (US);

Philip Alexander Shoemaker, Scotia, NY (US);

Erik Leeming Kvam, Albany, NY (US);

Xiaohui Chen, Niskayuna, NY (US);

Jaydeep Roy, Saratoga Springs, NY (US);

Assignee:

General Electric Company, Niskayuna, NY (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/077 (2010.01); C12M 1/40 (2006.01); C12M 1/00 (2006.01); B01F 5/02 (2006.01); C12M 1/02 (2006.01); B01F 5/00 (2006.01); B01F 5/10 (2006.01);
U.S. Cl.
CPC ...
C12M 45/09 (2013.01); B01F 5/0057 (2013.01); B01F 5/0206 (2013.01); B01F 5/0231 (2013.01); B01F 5/0256 (2013.01); B01F 5/10 (2013.01); C12M 27/00 (2013.01); C12M 47/04 (2013.01); C12N 5/0653 (2013.01); C12N 2501/70 (2013.01); C12N 2501/734 (2013.01); C12N 2521/00 (2013.01);
Abstract

A method of processing an adipose tissue to collect adipose derived regenerative cells is provided, wherein the method comprises providing a vessel comprising a fluid jet mixer; introducing the adipose tissue into the vessel; introducing a buffer solution into the vessel; washing the adipose tissue using the fluid jet mixer; introducing an enzyme solution into the vessel; initiating jet mixing into the vessel comprising the adipose tissue, the enzyme solution, and the buffer solution using the fluid jet mixer to digest the adipose tissue to form a digestion product; phase-separating the digestion product into a digested buoyant fat layer and a non-buoyant aqueous layer; and collecting the non-buoyant aqueous layer comprising the adipose derived regenerative cells. A system of processing an adipose tissue to collect adipose derived regenerative cells is also provided.


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