The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 18, 2015

Filed:

Sep. 23, 2011
Applicants:

Guillaume Durin, Boston, MA (US);

James A. Laugharn, Jr., Winchester, MA (US);

Inventors:

Guillaume Durin, Boston, MA (US);

James A. Laugharn, Jr., Winchester, MA (US);

Assignee:

Covaris, Inc., Woburn, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 1/28 (2006.01); B01L 3/00 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2006.01);
U.S. Cl.
CPC ...
B01L 3/50215 (2013.01); C12N 15/10 (2013.01); C12Q 1/6806 (2013.01); G01N 1/28 (2013.01); B01L 3/50255 (2013.01); B01L 2200/0631 (2013.01); B01L 2200/0663 (2013.01); B01L 2200/141 (2013.01); B01L 2300/042 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0838 (2013.01); B01L 2300/0841 (2013.01); B01L 2300/0854 (2013.01); B01L 2400/0409 (2013.01); B01L 2400/086 (2013.01); Y10T 436/25 (2015.01);
Abstract

Systems and methods include processing devices used to fragment nucleic acids to average nucleic acid sizes ranging from between about 5 kbp and about 20 kbp. A processing device may include an inlet portion and a channel upstream from a shearing region arranged so that a relatively constant pressure is established and maintained (e.g., a pressure that changes by less than about 40%) at an entrance of the shearing region during a majority of sample flow through the shearing region. In some embodiments, after forcing the sample through the shearing region once, the processing device may be taken out of the centrifuge, inverted and placed back into the centrifuge so that the sample is forced through the shearing region again.


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