The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 11, 2015

Filed:

May. 30, 2013
Applicant:

Ventana Medical Systems, Inc., Tucson, AZ (US);

Inventors:

Jerry W. Kosmeder, II, Tucson, AZ (US);

Casey A. Kernag, Tucson, AZ (US);

Donald Johnson, Oro Valley, AZ (US);

Christopher Bieniarz, Tucson, AZ (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/542 (2006.01); C07K 16/00 (2006.01); G01N 33/532 (2006.01); A61K 47/48 (2006.01); A61K 49/00 (2006.01); G01N 33/543 (2006.01); G01N 33/58 (2006.01); C07K 17/08 (2006.01); G01N 33/544 (2006.01); G01N 33/545 (2006.01); G01N 33/569 (2006.01); G01N 33/574 (2006.01); C12Q 1/68 (2006.01); G01N 33/566 (2006.01);
U.S. Cl.
CPC ...
G01N 33/532 (2013.01); A61K 47/48692 (2013.01); A61K 49/0058 (2013.01); A61K 49/0067 (2013.01); C07K 17/08 (2013.01); G01N 33/544 (2013.01); G01N 33/545 (2013.01); G01N 33/54353 (2013.01); G01N 33/56983 (2013.01); G01N 33/57492 (2013.01); G01N 33/581 (2013.01); G01N 33/582 (2013.01); G01N 33/587 (2013.01);
Abstract

Certain disclosed embodiments of the present invention concern the synthesis, derivatization, conjugation to immunoglobulins and signal amplification based on discrete, relatively short polymers having plural reactive functional groups that react with plural molecules of interest. Reactive functional groups, such as hydrazides, may be derivatized with a variety of detectable labels, particularly haptens. The remaining reactive functional groups may be conjugated directly to a specific binding molecule, such as to the oxidized carbohydrate of the Fc region of the antibody. Disclosed conjugates display large signal amplification as compared to those based on molecules derivatized with single haptens, and are useful for assay methods, particularly multiplexed assays.


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