The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 11, 2015
Filed:
Dec. 15, 2011
Megumi Kurokawa, Gunma, JP;
Keina Yamaguchi, Gunma, JP;
Risa Ogawa, Gunma, JP;
Masayoshi Tsukahara, Gunma, JP;
Koichi Kawakami, Shizuoka, JP;
Yoko Hayashi, Gunma, JP;
Megumi Kurokawa, Gunma, JP;
Keina Yamaguchi, Gunma, JP;
Risa Ogawa, Gunma, JP;
Masayoshi Tsukahara, Gunma, JP;
Koichi Kawakami, Shizuoka, JP;
Yoko Hayashi, Gunma, JP;
Abstract
The present invention relates to a method for integrating a gene fragment inserted between a pair of transposon sequences into a chromosome of a mammalian cell, comprising introducing at least one expression vector which comprises a gene fragment comprising a DNA encoding a protein of interest and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; and a method for producing the protein of interest comprising suspension-culturing a suspension mammalian cell which produces the protein of interest; and an a suspension mammalian cell which expresses the protein of interest.