The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 04, 2015

Filed:

Sep. 28, 2010
Applicants:

Ricardo E. Dolmetsch, Stanford, CA (US);

Masayuki Yazawa, Palo Alto, CA (US);

Inventors:

Ricardo E. Dolmetsch, Stanford, CA (US);

Masayuki Yazawa, Palo Alto, CA (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/11 (2006.01); C12N 15/62 (2006.01); C12N 15/70 (2006.01); C12N 15/79 (2006.01); C12N 15/00 (2006.01); G01N 33/50 (2006.01); A61K 38/00 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5008 (2013.01); C12N 15/00 (2013.01); C07K 2319/70 (2013.01);
Abstract

Compositions and methods for light controlled protein-protein interactions in a living cell. Two interacting PICL (protein interaction controlled by light) polypeptides are provided. The first polypeptide comprises an LOV (Light, Oxygen or Voltage) domain, which domain is a light sensor that uses flavin mononucleotide (FMN) as a chromophore. The second polypeptide, specifically interacts with the L polypeptide upon light activation of the LOV domain. One or both of the polypeptides are fused to a cellular protein of interest. Upon exposure to light, a targeted interaction between cellular proteins occurs. The ability to regulate protein-protein interactions with subcellular resolution using light is useful for controlling biochemical processes such transcription, receptor activation, protein degradation, synapse formation, etc. in cells and animals.


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