The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 04, 2015

Filed:

Nov. 24, 2009
Applicants:

Anurag Khetan, Lexington, MA (US);

Yao-ming Huang, San Diego, CA (US);

Jana Dolnikova, Waltham, MA (US);

Nels Pederson, Mansfield, MA (US);

Helena Yusuf-makagiansar, San Diego, CA (US);

Paul Chen, Belmont, MA (US);

Thomas Ryll, Lexington, MA (US);

Inventors:

Anurag Khetan, Lexington, MA (US);

Yao-ming Huang, San Diego, CA (US);

Jana Dolnikova, Waltham, MA (US);

Nels Pederson, Mansfield, MA (US);

Helena Yusuf-Makagiansar, San Diego, CA (US);

Paul Chen, Belmont, MA (US);

Thomas Ryll, Lexington, MA (US);

Assignee:

BIOGEN MA INC., Cambridge, MA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07K 14/435 (2006.01); C12N 5/00 (2006.01); C12N 5/02 (2006.01); C12N 5/071 (2010.01); C12N 15/00 (2006.01); C12N 15/06 (2006.01); C12N 15/09 (2006.01); C12P 21/02 (2006.01); C07K 16/24 (2006.01);
U.S. Cl.
CPC ...
C12P 21/02 (2013.01); C07K 16/241 (2013.01); C07K 16/24 (2013.01); C07K 2317/41 (2013.01); C07K 2317/515 (2013.01); C12N 2500/32 (2013.01); C12N 2510/02 (2013.01);
Abstract

The present invention relates to methods of reducing substitution of amino acids during the production of polypeptides of interest in mammalian cells. By varying the concentration of amino acids in the culture medium, heterogeneity of the polypeptide's amino acid sequence is decreased, leading to higher yields of functional protein.


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