The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 04, 2015

Filed:

May. 21, 2007
Applicants:

Marie J. Archer, Alexandria, VA (US);

Baochuan Lin, Bethesda, MD (US);

David a Stenger, Herndon, VA (US);

Inventors:

Marie J. Archer, Alexandria, VA (US);

Baochuan Lin, Bethesda, MD (US);

David A Stenger, Herndon, VA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C12N 15/10 (2006.01); C40B 30/04 (2006.01); C40B 40/08 (2006.01); C40B 50/18 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1006 (2013.01); C12N 15/1013 (2013.01); C12Q 1/68 (2013.01); C40B 30/04 (2013.01); C40B 40/08 (2013.01); C40B 50/18 (2013.01); C07B 2200/11 (2013.01);
Abstract

A method of: providing a solid surface having a dendrimer molecule bound thereto and a single-stranded probe nucleic acid immobilized to the dendrimer; contacting the solid surface with a sample suspected or known to contain a double-stranded complimentary target nucleic acid; denaturing the target nucleic acids at thermal conditions and in a salt concentration sufficient to denature the target nucleic acids to produce denatured nucleic acids; and cooling the sample to allow hybridization of the denatured nucleic acids to the probe nucleic acids. An article having: one or more paramagnetic microbeads; a dendrimer molecule bound to the beads; and a probe nucleic acid immobilized to the dendrimer.


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