The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 04, 2015

Filed:

Aug. 31, 2010
Applicants:

Darren W. Branch, Albuquerque, NM (US);

Erika Jane Cooley, Albuquerque, NM (US);

Gennifer Tanabe Smith, Albuquerque, NM (US);

Conrad D. James, Albuquerque, NM (US);

Jaime L. Mcclain, Albuquerque, NM (US);

Inventors:

Darren W. Branch, Albuquerque, NM (US);

Erika Jane Cooley, Albuquerque, NM (US);

Gennifer Tanabe Smith, Albuquerque, NM (US);

Conrad D. James, Albuquerque, NM (US);

Jaime L. McClain, Albuquerque, NM (US);

Assignee:

Sandia Corporation, Albuquerque, NM (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/42 (2006.01); C12M 1/00 (2006.01); C12N 1/06 (2006.01); B01F 13/00 (2006.01); B01F 11/02 (2006.01); B01J 19/10 (2006.01); G01N 35/00 (2006.01); C12M 3/06 (2006.01);
U.S. Cl.
CPC ...
C12M 47/06 (2013.01); C12N 1/066 (2013.01); B01F 11/0241 (2013.01); B01F 13/0059 (2013.01); B01J 19/10 (2013.01); B01L 2400/0439 (2013.01); C12M 23/16 (2013.01); G01N 2035/00554 (2013.01);
Abstract

A microfluidic acoustic-based cell lysing device that can be integrated with on-chip nucleic acid extraction. Using a bulk acoustic wave (BAW) transducer array, acoustic waves can be coupled into microfluidic cartridges resulting in the lysis of cells contained therein by localized acoustic pressure. Cellular materials can then be extracted from the lysed cells. For example, nucleic acids can be extracted from the lysate using silica-based sol-gel filled microchannels, nucleic acid binding magnetic beads, or Nafion-coated electrodes. Integration of cell lysis and nucleic acid extraction on-chip enables a small, portable system that allows for rapid analysis in the field.


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