The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 28, 2015
Filed:
Sep. 09, 2013
Sukanta Bhattacharyya, Belmont, CA (US);
Daniel Sobek, Portola Valley, CA (US);
Sukanta Bhattacharyya, Belmont, CA (US);
Daniel Sobek, Portola Valley, CA (US);
Zymera, Inc., San Jose, CA (US);
Abstract
A qualitative and quantitative hydrolase detection method includes providing a caged coelenterazine molecule having a detection molecule bonded between a first bonding site or a second bonding site of a coelenterazine molecule and a steric caging group; activating an uncaged coelenterazine molecule by cleaving the detection molecule in the caged coelenterazine molecule with a hydrolase enzyme; determining a light emission from a coelenterazine-activated bioluminescent enzyme reacting with the uncaged coelenterazine molecule activated in the presence of the hydrolase enzyme; and correlating the light emission for quantitatively and qualitatively determining the presence of the hydrolase enzyme.