The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 28, 2015

Filed:

Aug. 30, 2011
Applicants:

Hiroshi Ban, Tsukuba, JP;

Yasuji Ueda, Tsukuba, JP;

Noemi Fusaki, Tsukuba, JP;

Koichi Saeki, Tsukuba, JP;

Mamoru Hasegawa, Tsukuba, JP;

Inventors:

Hiroshi Ban, Tsukuba, JP;

Yasuji Ueda, Tsukuba, JP;

Noemi Fusaki, Tsukuba, JP;

Koichi Saeki, Tsukuba, JP;

Mamoru Hasegawa, Tsukuba, JP;

Assignee:

DNAVEC CORPORATION, Ibaraki, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/02 (2006.01); C12N 15/86 (2006.01); C12N 5/074 (2010.01); C12N 15/63 (2006.01);
U.S. Cl.
CPC ...
C12N 15/86 (2013.01); C12N 5/0696 (2013.01); C12N 15/63 (2013.01); C12N 2501/602 (2013.01); C12N 2501/603 (2013.01); C12N 2501/604 (2013.01); C12N 2506/13 (2013.01); C12N 2510/00 (2013.01); C12N 2760/18843 (2013.01);
Abstract

The present invention provides Sendai virus vectors in which genes that encode reprograming factors for inducing pluripotent stem cells are incorporated in a specific order, compositions comprising these vectors for gene delivery to be used in the induction of pluripotent stem cells, and uses thereof. Incorporation of the KLF gene, OCT gene, and SOX gene in a specific order into a single Sendai virus vector successfully and significantly increased the efficiency of pluripotent stem cell induction. Loading multiple reprogramming factors into a single vector can further increase the induction efficiency of pluripotent stem cells while reducing the number of necessary vectors.


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