The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 28, 2015

Filed:

Dec. 14, 2009
Applicants:

William R. Farmer, Concord, MA (US);

Jeff Bickmeier, Arlington, MA (US);

Chenfeng LU, Watertown, MA (US);

Dong-eun Chang, Newton, MA (US);

Frank Skraly, Watertown, MA (US);

Thomas Martin Ramseier, Newton, MA (US);

Inventors:

William R. Farmer, Concord, MA (US);

Jeff Bickmeier, Arlington, MA (US);

Chenfeng Lu, Watertown, MA (US);

Dong-Eun Chang, Newton, MA (US);

Frank Skraly, Watertown, MA (US);

Thomas Martin Ramseier, Newton, MA (US);

Assignee:

Metabolix, Inc., Cambridge, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/20 (2006.01); C12N 1/32 (2006.01); C12P 7/18 (2006.01); C12P 13/08 (2006.01); C12P 7/62 (2006.01); C12P 7/46 (2006.01); C07H 21/04 (2006.01); C07C 31/18 (2006.01); C12N 15/52 (2006.01); C12N 9/04 (2006.01); C12N 9/02 (2006.01); C12N 9/10 (2006.01); C12N 9/80 (2006.01); C12N 9/88 (2006.01); C12N 9/00 (2006.01); C12P 7/04 (2006.01); C12P 7/42 (2006.01); C12P 7/44 (2006.01);
U.S. Cl.
CPC ...
C12N 15/52 (2013.01); C12N 9/0006 (2013.01); C12N 9/0008 (2013.01); C12N 9/0069 (2013.01); C12N 9/10 (2013.01); C12N 9/1029 (2013.01); C12N 9/1096 (2013.01); C12N 9/80 (2013.01); C12N 9/88 (2013.01); C12N 9/93 (2013.01); C12P 7/04 (2013.01); C12P 7/18 (2013.01); C12P 7/42 (2013.01); C12P 7/44 (2013.01); C12P 7/625 (2013.01);
Abstract

Recombinant hosts for producing polyhydroxyalkanoates and methods of producing polyhydroxyalkanoates from renewable carbon substrates are provided. Certain recombinant hosts that produce 5 carbon chemicals such as 5-aminopentanoate (5AP), 5-hydroxyvalerate (5HV), glutarate, and 1,5 pentanediol (PDO) are also provided. One embodiment provides a recombinant host expressing a gene encoding a heterologous enzyme selected from the group consisting of a polyhydroxyalkanoate synthase and a 5-hydroxyvalerate-CoA (5HV-CoA) transferase, wherein the host produces a polymer containing 5-hydroxyvalerate. Preferably, the host expresses both a polyhydroxyalkanoate synthase and a 5HV-CoA transferase. The host can be prokaryotic or eukaryotic. A preferred prokaryotic host is. The polymers produced by the recombinant hosts can be homopolymers or copolymers of 5-hydroxyvalerate. A preferred copolymer is poly(3-hydroxybutyrate-co-5-hydroxyvalerate).


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