The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 07, 2015
Filed:
Feb. 08, 2012
Applicants:
Gusti Zeiner, San Mateo, CA (US);
Robert A. Ach, San Francisco, CA (US);
Inventors:
Gusti Zeiner, San Mateo, CA (US);
Robert A. Ach, San Francisco, CA (US);
Assignee:
Agilent Technologies, Inc., Santa Clara, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/04 (2006.01); C12N 15/10 (2006.01); C12N 15/66 (2006.01); C12N 9/00 (2006.01); C12P 19/34 (2006.01);
U.S. Cl.
CPC ...
C12N 15/10 (2013.01); C12N 15/66 (2013.01); C12N 9/93 (2013.01); C12Y 605/01004 (2013.01); C12P 19/34 (2013.01);
Abstract
A method of processing an RNA sample is provided. In certain embodiments, the method may comprise: a) obtaining a fragmented RNA sample comprising: i. RNA fragments of long RNA molecules; and ii. unfragmented short RNA; and b) contacting said fragmented RNA sample with a first adaptor in the presence of a RtcB ligase, thereby producing a ligated RNA sample comprising adaptor-ligated fragments of long RNA. A kit for performing the method is also provided.