The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 30, 2015
Filed:
Sep. 22, 2011
Min-hsien Wu, Kaohsiung, TW;
Shih-siou Wang, Kaohsiung, TW;
Wan-chen Tsai, Taichung, TW;
Yu-han Chang, Taoyuan, TW;
Chia-hsun Hsieh, Taoyuan, TW;
Yen-ting Liu, Kaohsiung, TW;
Zhanfeng Cui, Taoyuan, TW;
Min-Hsien Wu, Kaohsiung, TW;
Shih-Siou Wang, Kaohsiung, TW;
Wan-Chen Tsai, Taichung, TW;
Yu-Han Chang, Taoyuan, TW;
Chia-Hsun Hsieh, Taoyuan, TW;
Yen-Ting Liu, Kaohsiung, TW;
Zhanfeng Cui, Taoyuan, TW;
Chang Gung University, Taoyuan, TW;
Abstract
A microfluidic chip for three-dimensional cell culture with high-throughput perfusion includes an array of cell culture units, each unit including a cell culture medium inlet hole connecting to one cell culture medium tank, at least one micro-bioreactor, at least one microchannel and at least one medium collection and analysis tank. Each medium collection and analysis tank is connected to an air chamber with an air channel and the air chamber has negative pressure source holes to generate negative pressure to drive the culture medium. The microfluidic chip also includes an intermediate plate connected to the bottom surface of the roof, and two bottom plates detachably assembled at the bottom of the intermediate plate. The first and second bottom surfaces have micro-bioreactors and cylindrical recessed slots and the intermediate plate has corresponding holes to achieve the goal of three-dimensional cell culture using minimum experimental resources with high-throughput perfusion.