The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 23, 2015

Filed:

May. 21, 2010
Applicants:

Katsuhiko Kitsugi, Chiba, JP;

Satoru Sugita, Chiba, JP;

Noriyuki Ohnishi, Chiba, JP;

Inventors:

Katsuhiko Kitsugi, Chiba, JP;

Satoru Sugita, Chiba, JP;

Noriyuki Ohnishi, Chiba, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/543 (2006.01); G01N 33/58 (2006.01); G01N 21/78 (2006.01); G01N 33/542 (2006.01);
U.S. Cl.
CPC ...
G01N 33/583 (2013.01); G01N 21/78 (2013.01); G01N 33/542 (2013.01); G01N 33/582 (2013.01);
Abstract

Provided are a detection method and a quantification method for a detection target, which can detect and quantify the detection target rapidly, inexpensively, simply, and with high accuracy in a variety of environments. A method for detecting the detection target in a specimen includes processes in which there are mixed a first binding substance which binds a first material containing a stimuli-responsive polymer and a first affinity substance which has affinity for the detection target, a second binding substance which binds a hydrophilic second material and a second affinity substance which has affinity for the detection target, and a specimen; the resulting mixture is placed under conditions where the stimuli-responsive polymer agglutinates and the mixture is developed into a developing carrier, or the resulting mixture is developed into a developing carrier and the mixture is placed under conditions where the stimuli-responsive polymer agglutinates; the signal produced by the presence of the first binding substance or the second binding substance in the developing carrier is verified; and the presence of the detection target in the specimen is determined when the signal is different from that when the detection target is absent. The first affinity substance and the second affinity substance can bind to different positions of the detection target.


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