The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 23, 2015

Filed:

Aug. 04, 2011
Applicants:

Atsushi Ichiyanagi, Chiba, JP;

Kozo Hirokawa, Chiba, JP;

Yasuko Tanabe, Chiba, JP;

Yosuke Masakari, Chiba, JP;

Inventors:

Atsushi Ichiyanagi, Chiba, JP;

Kozo Hirokawa, Chiba, JP;

Yasuko Tanabe, Chiba, JP;

Yosuke Masakari, Chiba, JP;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/06 (2006.01); C12N 1/00 (2006.01); C12N 15/63 (2006.01); G01N 33/72 (2006.01); C12Q 1/26 (2006.01);
U.S. Cl.
CPC ...
C12N 9/0032 (2013.01); C12N 1/00 (2013.01); C12N 15/63 (2013.01); G01N 33/723 (2013.01); G01N 2333/902 (2013.01); C12Q 1/26 (2013.01);
Abstract

This invention provides an amadoriase having high substrate specificity to fructosyl valyl histidine. Such amadoriase comprises substitution of one or more amino acid residues at positions corresponding to amino acids selected from the group consisting of position 98, position 259, position 154, position 125, position 261, position 263, position 106, position 103, position 355, position 96, position 66, position 67, position 70, position 100, position 110, position 113, position 114, and position 156 in the amadoriase derived from the genus. This invention enables accurate measurement of α-fructosyl valyl histidine derived from the β-chain amino terminus in glycated hemoglobin in the presence of ε-fructosyl lysine.


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