The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 16, 2015

Filed:

Oct. 14, 2009
Applicants:

David Erickson, Ithaca, NY (US);

Yun Suk Huh, Daejeon, KR;

Carl A. Batt, Groton, NY (US);

Adam Joseph Lowe, Syracuse, NY (US);

Inventors:

David Erickson, Ithaca, NY (US);

Yun Suk Huh, Daejeon, KR;

Carl A. Batt, Groton, NY (US);

Adam Joseph Lowe, Syracuse, NY (US);

Assignee:

Cornell University, Ithaca, NY (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C12P 19/34 (2006.01); C07H 21/00 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01); B01F 13/00 (2006.01); B01L 3/00 (2006.01); G01N 21/65 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6827 (2013.01); B01F 13/0076 (2013.01); B01L 3/50273 (2013.01); B01L 3/502761 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/0809 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/0887 (2013.01); B01L 2400/0415 (2013.01); B01L 2400/0424 (2013.01); C12Q 1/6862 (2013.01); C12Q 2565/632 (2013.01); G01N 21/658 (2013.01);
Abstract

A method for detecting target nucleic acids such as SNPs is provided. The method comprises performing a ligase detection reaction (LDR), performing surface enhanced Raman scattering (SERS) on the LDR, and analyzing the outcome of the LDR using analysis and/or quantification of the SERS by detecting an emitted Raman signature. The LDR-SERS method can be used for sensitive and specific detection of any nucleic acid sequence of interest. A microfluidic SERS detection device is also provided. The device comprises electrokinetically active microwells for mixing and concentrating analytes and in which analytes can be quantified. The device can be used for performing the LDR-SERS method in optofluidic chip format.


Find Patent Forward Citations

Loading…