The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 16, 2015

Filed:

Jan. 13, 2012
Applicants:

Melissa Tuchscherer, Kansas City, MO (US);

Perry Kilpatrick, Kansas City, MO (US);

Richard Slawecki, Warminster, PA (US);

Fernando Manuel Rubio, Doylestown, PA (US);

Inventors:

Melissa Tuchscherer, Kansas City, MO (US);

Perry Kilpatrick, Kansas City, MO (US);

Richard Slawecki, Warminster, PA (US);

Fernando Manuel Rubio, Doylestown, PA (US);

Assignee:

Abraxis LLC, Warminster, PA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/46 (2006.01); C12Q 1/34 (2006.01); G01N 31/22 (2006.01); G01N 33/573 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/46 (2013.01); G01N 31/22 (2013.01); C12Q 2334/00 (2013.01);
Abstract

The present invention provides a kit and a method of testing for carbamates in amine cured epoxies. The method and kit are based on the use of an enzyme that is inhibited by carbamates. A specific embodiment of the kit includes acetylthiocholine (ATC), dithio-bis (2-nitrobenzoic acid) (DTNB), and acetyl-cholinesterase (ACh-E). In the corresponding method embodiment, the method comprises the steps of collecting carbamates from the epoxy, adding the collected carbamates to a solution, adding acetyl-cholinesterase (ACh-E) to the solution, adding acetylthiocholine (ATC) to the solution, adding dithio-bis (2-nitrobenzoic acid) (DTNB) to the solution, and ultimately measuring the intensity of the yellow color of the solution.


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