The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 09, 2015

Filed:

May. 19, 2009
Applicants:

Hirofumi Doi, Chiba, JP;

Masahiro Matsuoka, Tokyo, JP;

Tatsuhiro Nakano, Chiba, JP;

Chizuru Nagayoshi, Chiba, JP;

Inventors:

Hirofumi Doi, Chiba, JP;

Masahiro Matsuoka, Tokyo, JP;

Tatsuhiro Nakano, Chiba, JP;

Chizuru Nagayoshi, Chiba, JP;

Assignee:

CELISH FD, INC., Chiba, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); G01N 33/53 (2006.01); C12M 1/34 (2006.01); G01N 21/64 (2006.01); G01N 31/22 (2006.01); C07H 21/04 (2006.01); C07H 21/02 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6832 (2013.01); C12Q 1/6841 (2013.01);
Abstract

A method for identifying the presence of a target gene mRNA is provided, which involves hybridizing one or more oligonucleic acid probes with the target gene mRNA expressed in a tissue sample, and detecting a low-molecular-weight compound label added to at least one of the bases of the oligonucleic acid probes. The oligonucleic acid probes are contacted with the tissue sample for hybridization with the target gene mRNA after pretreating (prehybridizing) one or more dummy oligonucleic acids with the tissue sample, or a mixture of the oligonucleic acid probes and the dummy oligonucleic acids is contacted with the sample tissue to hybridize the oligonucleic acid probes with the target gene mRNA.


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