The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 09, 2015

Filed:

Jul. 25, 2012
Applicants:

Takehide Kimura, Tokyo, JP;

Kazuhito Miyauchi, Tokyo, JP;

Hideyuki Kuwata, Shizuoka, JP;

Inventors:

Takehide Kimura, Tokyo, JP;

Kazuhito Miyauchi, Tokyo, JP;

Hideyuki Kuwata, Shizuoka, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/44 (2006.01); C12Q 1/61 (2006.01); G01N 33/92 (2006.01); C12Q 1/28 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/61 (2013.01); G01N 33/92 (2013.01); C12Q 1/28 (2013.01); C12Q 1/44 (2013.01); C12Y 101/03017 (2013.01); C12Y 301/01005 (2013.01); C12Y 301/04004 (2013.01);
Abstract

Provided is a method for simply and accurately measuring sphingomyelin in a sample, and a kit therefore. The method is a method for measuring sphingomyelin in a sample, comprising reacting the sample with a phospholipase D which does not react with sphingomyelin and lysophosphatidylcholine but reacts with phosphatidylcholine, a lysophospholipase or a monoglyceride lipase, and a choline oxidase, eliminating the formed hydrogen peroxide, reacting the resultant with a phospholipase D which does not react with glycerol-3-phosphorylcholine and free fatty acid but reacts with sphingomyelin, and a choline oxidase, and measuring the formed hydrogen peroxide.


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