The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 02, 2015

Filed:

Aug. 08, 2013
Applicant:

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

Timothy J. Denison, Andover, MA (US);

Alexis Sauer-Budge, Brighton, MA (US);

Jene A. Golovchenko, Lexington, MA (US);

Amit Meller, Newton, MA (US);

Eric Brandin, Portsmouth, NH (US);

Daniel Branton, Lexington, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 27/447 (2006.01); C12Q 1/68 (2006.01); G01N 33/487 (2006.01);
U.S. Cl.
CPC ...
G01N 27/447 (2013.01); C12Q 1/6869 (2013.01); G01N 33/48721 (2013.01);
Abstract

The invention relates to a method for characterizing a target polynucleic acid by providing a surface containing a channel of a dimension sufficient to allow sequential monomer-by-monomer passage of a single-stranded polynucleic acid, but not of a double-stranded polynucleic acid; providing a source of hybridized target polynucleic acid at the surface; inducing passage of the target polynucleic acid through the channel, whereby the target polynucleic acid undergoes base pair separation (melts) prior to its passage; and making one or more measurements over time as the target polynucleic acid moves relative to the channel yielding data suitable to determine a monomer-dependent characteristic of the target polynucleic acid.


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