The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 02, 2015

Filed:

Nov. 25, 2004
Applicants:

Paulo Eduardo Mantelatto, Piracicaba, BR;

Alvaro Minto Duzzi, Serrana, BR;

Tetuhiko Sato, Sao Paulo, BR;

Nazareno Antonio Sertori Durao, Brodowski, BR;

Roberto Vianna Nonato, Sao Paulo, BR;

Carlo Rocchiccioli, Sao Paulo, BR;

Sonia Maria Kesserlingh, Sertaozinho, BR;

Inventors:

Paulo Eduardo Mantelatto, Piracicaba, BR;

Alvaro Minto Duzzi, Serrana, BR;

Tetuhiko Sato, Sao Paulo, BR;

Nazareno Antonio Sertori Durao, Brodowski, BR;

Roberto Vianna Nonato, Sao Paulo, BR;

Carlo Rocchiccioli, Sao Paulo, BR;

Sonia Maria Kesserlingh, Sertaozinho, BR;

Assignee:

PHB Industrial S.A., Sao Paulo, BR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C08G 63/90 (2006.01); C08G 63/89 (2006.01); C12P 7/62 (2006.01); C08G 63/06 (2006.01);
U.S. Cl.
CPC ...
C08G 63/90 (2013.01); C08G 63/06 (2013.01); C08G 63/89 (2013.01); C12P 7/625 (2013.01);
Abstract

A process for recovering polyhydroxyalkanoates (PHAs) from cellular biomass of bacteria, said biomass being obtained by fermentation and in the form of a cellular biomass slurry in aqueous suspension, comprising the steps of: submitting the slurry to operations of injection of PHA solvent, agitation and heating, in order to form a suspension comprising PHA solvent with the dissolved PHA, water and insoluble residues; recuperating the solvent enriched with PHA; rapidly cooling the solution of PHA solvent to precipitate the dissolved PHA; micro-filtrating the suspension of PHA precipitated in the solvent, in order to separate a paste concentrated with precipitated PHA; washing with water, heating and agitating the concentrated PHA paste, to promote evaporation of the solvent and to obtain a suspension containing PHA granules; agitating and shearing the PHA granules and depleting the residual solvent; and separating the purified PHA particles from the suspension.


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