The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 26, 2015

Filed:

Jul. 01, 2009
Applicants:

Sergey I. Ivashuta, Ballwin, MO (US);

Barbara E. Wiggins, Chesterfield, MO (US);

Yuanji Zhang, Weldon Spring, MO (US);

Inventors:

Sergey I. Ivashuta, Ballwin, MO (US);

Barbara E. Wiggins, Chesterfield, MO (US);

Yuanji Zhang, Weldon Spring, MO (US);

Assignee:

Monsanto Technology LLC, St. Louis, MO (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/113 (2010.01); C12N 15/00 (2006.01); C12N 15/11 (2006.01); C12N 15/82 (2006.01);
U.S. Cl.
CPC ...
C12N 15/111 (2013.01); C12N 15/8216 (2013.01); C12N 15/8218 (2013.01); C12N 15/8261 (2013.01); C12N 2310/141 (2013.01);
Abstract

This invention provides recombinant DNA constructs and methods for manipulating expression of a target gene that is regulated by a small RNA, by interfering with the binding of the small RNA to its target gene. More specifically, this invention discloses recombinant DNA constructs encoding cleavage blockers, 5-modified cleavage blockers, and translational inhibitors useful for modulating expression of a target gene and methods for their use. Further disclosed are miRNA targets useful for designing recombinant DNA constructs including miRNA-unresponsive transgenes, miRNA decoys, cleavage blockers, 5-modified cleavage blockers, and translational inhibitors, as well as methods for their use, and transgenic eukaryotic cells and organisms containing such constructs.


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