The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 26, 2015

Filed:

Apr. 26, 2012
Applicants:

M. Peter Marinkovich, Redwood City, CA (US);

Alfred T. Lane, Los Altos, CA (US);

Jayakumar Rajadas, Cupertino, CA (US);

Inventors:

M. Peter Marinkovich, Redwood City, CA (US);

Alfred T. Lane, Los Altos, CA (US);

Jayakumar Rajadas, Cupertino, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
A61K 38/39 (2006.01); A61M 5/00 (2006.01); C07K 14/78 (2006.01); C12N 9/02 (2006.01); A61M 37/00 (2006.01); C12N 15/67 (2006.01);
U.S. Cl.
CPC ...
A61M 37/0015 (2013.01); A61K 38/39 (2013.01); C07K 14/78 (2013.01); C12N 15/67 (2013.01); C12N 9/0071 (2013.01);
Abstract

Improved methods are provided for the recombinant synthesis of collagen, particularly collagen VII, in host cell, and for therapeutic delivery of the same. The recombinant collagen is produced in a host cell that has increased levels of prolyl-4-hydroxylase, relative to basal cell levels. The collagen produced by the methods of the invention has increased numbers of modified proline residues, relative to a recombinant collagen produced in a host cell having basal levels of prolyl-4-hydroxylase. The increased proline modification provides for a collagen having increased stability, including increased in vivo stability.


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