The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 26, 2015
Filed:
Apr. 27, 2011
Julia Ashworth-sharpe, Tucson, AZ (US);
Chol Steven Yun, Tucson, AZ (US);
Zhanna Zhilina, Tucson, AZ (US);
Adrian E. Murillo, Tucson, AZ (US);
Donald D. Johnson, Oro Valley, AZ (US);
Michael Farrell, Tucson, AZ (US);
Jerome W. Kosmeder, Tucson, AZ (US);
Christopher Bieniarz, Tucson, AZ (US);
Julia Ashworth-Sharpe, Tucson, AZ (US);
Chol Steven Yun, Tucson, AZ (US);
Zhanna Zhilina, Tucson, AZ (US);
Adrian E. Murillo, Tucson, AZ (US);
Donald D. Johnson, Oro Valley, AZ (US);
Michael Farrell, Tucson, AZ (US);
Jerome W. Kosmeder, Tucson, AZ (US);
Christopher Bieniarz, Tucson, AZ (US);
Ventana Medical Systems, Inc., Tucson, AZ (US);
Abstract
Disclosed herein are antibody-nanoparticle conjugates that include two or more nanoparticles (such as gold, palladium, platinum, silver, copper, nickel, cobalt, iridium, or an alloy of two or more thereof) directly linked to an antibody or fragment thereof through a metal-thiol bond. Methods of making the antibody-nanoparticle conjugates disclosed herein include reacting an arylphosphine-nanoparticle composite with a reduced antibody to produce an antibody-nanoparticle conjugate. Also disclosed herein are methods for detecting a target molecule in a sample that include using an antibody-nanoparticle conjugate (such as the antibody-nanoparticle conjugates described herein) and kits for detecting target molecules utilizing the methods disclosed herein.