The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 26, 2015

Filed:

Jun. 23, 2011
Applicants:

Leopoldo Della Ciana, Bologna, IT;

Federica Rodeghiero, Bologna, IT;

Rossana Perciaccante, Bologna, IT;

Inventors:

Leopoldo Della Ciana, Bologna, IT;

Federica Rodeghiero, Bologna, IT;

Rossana Perciaccante, Bologna, IT;

Assignee:

Cyanagen S.R.L., Bologna, IT;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 21/76 (2006.01); G01N 33/53 (2006.01); G01N 33/58 (2006.01); C12Q 1/28 (2006.01);
U.S. Cl.
CPC ...
G01N 33/582 (2013.01); C12Q 1/28 (2013.01); G01N 33/5306 (2013.01);
Abstract

Method for increasing and regulating the emission of light from a chemiluminescent reaction including luminol, a peroxidase enzyme, an oxidant and an electron mediator (primary enhancer) through the use of an acylation catalyst (secondary enhancer) belonging to the class of N-azoles, i.e., a class of five-membered nitrogen heteroaromatic ring compounds containing at least one other atom of nitrogen. N-azoles, which are especially useful as secondary enhancers are imidazole, 1-methylimidazole, 1,2,3-triazole and 1,2,4-triazole. The invention also describes the use in diagnostic assays of chemiluminescent substrates containing said N-azoles, as secondary enhancers.


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