The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 19, 2015

Filed:

Dec. 08, 2010
Applicants:

Gerd Rundström, Uppsala, SE;

Per Matsson, Knivsta, SE;

Paul Christopher, Pontypridd, GB;

Inventors:

Gerd Rundström, Uppsala, SE;

Per Matsson, Knivsta, SE;

Paul Christopher, Pontypridd, GB;

Assignee:

Phadia AB, Uppsala, SE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/558 (2006.01); C12M 1/34 (2006.01); G01N 33/543 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
G01N 33/558 (2013.01); G01N 33/543 (2013.01); G01N 33/6854 (2013.01); G01N 2800/24 (2013.01); Y10S 435/975 (2013.01); Y10S 436/807 (2013.01); Y10S 435/97 (2013.01); Y10S 436/81 (2013.01); Y10S 436/821 (2013.01); Y10S 435/805 (2013.01); Y10S 435/81 (2013.01); Y10S 435/973 (2013.01); Y10S 436/805 (2013.01); Y10S 436/808 (2013.01);
Abstract

A two step lateral flow assay method for identifying IgE antibodies in a sample comprises applying a sample to a sample port of a device, wherein the device is adapted to deliver the sample to a lateral flow matrix having a plurality of IgE antigen species immobilized at respective positions at a first location; allowing the sample to travel along the lateral flow matrix through the immobilized plurality of IgE antigen species to a second location downstream of the first location; and, after a predetermined period of time, applying liquid buffer to the lateral flow matrix to mobilize labeled reagent which is adapted to bind anti-IgE antibody and which is dried on the lateral flow matrix at a location upstream of the sample port delivery of the sample to the lateral flow matrix, and allowing labeled reagent mobilized by the liquid buffer to travel along the lateral flow matrix through the immobilized plurality of IgE antigen species and bind with any IgE antibody bound to the immobilized IgE antigen species, and to travel to a second location downstream of the first location where the mobilized labeled reagent causes a visible change to occur at the second location.


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