The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 19, 2015

Filed:

Nov. 26, 2012
Applicants:

Thierry Burnouf, Lille, FR;

Cheng-yao Su, Taipei, TW;

Inventors:

Thierry Burnouf, Lille, FR;

Cheng-Yao Su, Taipei, TW;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/08 (2006.01); A61K 35/16 (2006.01); C12N 5/00 (2006.01); A61K 38/18 (2006.01); A61K 38/30 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0018 (2013.01); A61K 38/1808 (2013.01); A61K 38/1841 (2013.01); A61K 38/30 (2013.01); A61K 35/19 (2013.01);
Abstract

The invention concerns human platelet extracts rich in growth factors (PGF) for wound healing and stem cell expansion. Accordingly the subject invention relates to a virally-inactivated growth factors-containing platelet lysate depleted of PDGF and VEGF, which is preferably enriched in TGF, IGF and EGF-rich. The present invention further concerns a method for obtaining a platelet lysate comprising the steps of contacting a starting platelet concentrate with a solvent and/or a detergent, incubating the starting platelet concentrate with the solvent and/or detergent for a period of at least 5 minutes to 6 hours, at a pH maintained in a range from about 6.0 to about 9.0, and at a temperature within the range of from 2° C. to 50° C., optionally removing the solvent and/or the detergent by oil extraction and obtaining an aqueous protein phase, and incubating the solvent and/or detergent-treated platelet concentrate or the aqueous protein phase with charcoal.


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