The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 19, 2015

Filed:

Jul. 27, 2012
Applicants:

Steven Kattman, Madison, WI (US);

Chad Koonce, Madison, WI (US);

Natsuyo Aoyama, Madison, WI (US);

Jennifer Luebke-wheeler, Baraboo, WI (US);

Brad Swanson, Waunakee, WI (US);

Inventors:

Steven Kattman, Madison, WI (US);

Chad Koonce, Madison, WI (US);

Natsuyo Aoyama, Madison, WI (US);

Jennifer Luebke-Wheeler, Baraboo, WI (US);

Brad Swanson, Waunakee, WI (US);

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 33/567 (2006.01); C12Q 1/00 (2006.01); C12N 5/077 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0657 (2013.01); C12N 2500/25 (2013.01); C12N 2500/32 (2013.01); C12N 2500/33 (2013.01); C12N 2500/36 (2013.01); C12N 2500/99 (2013.01);
Abstract

The present invention concerns methods and compositions for promoting a conversion in stem cells or cells derived therefrom from glycolysis to aerobic respiration and its associated pathways, including oxidative phosphorylation, the TCA cycle, fatty acid oxidation, and pyruvate decarboxylation, for example. In specific embodiments, certain media is employed to improve metabolic maturation of particular stem cell-derived cells, including human embryonic stem cells and human induced pluripotent stem cells, for example. Particular media comprises media without glucose or optionally without pyruvate, with fatty acids, with L-carnitine, with taurine, creatine, non-essential amino acids, L-glutamine, and with anti-oxidants or free-radical scavengers, for example.


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