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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 9017992 B1

PDFFull Text
Date of Patent:
Apr. 28, 2015

Filed:

Jun. 30, 2006

In situ hybridization detection method

Applicants:

Lars Winther, Smørum, DK;

Jesper Lohse, København NV, DK;

Susanne Gabs, Stenlille, DK;

Kenneth Heesche Petersen, Smørum, DK;

Inventors:

Lars Winther, Smørum, DK;

Jesper Lohse, København NV, DK;

Susanne Gabs, Stenlille, DK;

Kenneth Heesche Petersen, Smørum, DK;

Assignee:

Dako Denmark A/S, , DK;

Attorney:

Finnegan, Henderson, Farabow, Garrett & Dunner LLP

Primary Examiner:

Narayan Bhat

Int. Cl.
CPC ...
C12M 1/34 (2006.01); C12Q 1/68 (2006.01); C07H 21/02 (2006.01); B01L 3/00 (2006.01); G01N 31/22 (2006.01); G01N 33/52 (2006.01); A61K 47/48 (2006.01); A61K 49/00 (2006.01); C07H 19/06 (2006.01); C07H 19/10 (2006.01); C07H 19/16 (2006.01); C07H 19/20 (2006.01); C07H 21/00 (2006.01); C08G 69/02 (2006.01); C07C 231/02 (2006.01); C07C 233/36 (2006.01); G01N 33/53 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6804 (2013.01); A61K 47/4823 (2013.01); A61K 47/48538 (2013.01); A61K 47/48715 (2013.01); A61K 49/0041 (2013.01); A61K 49/0043 (2013.01); A61K 49/0056 (2013.01); C07H 19/06 (2013.01); C07H 19/10 (2013.01); C07H 19/16 (2013.01); C07H 19/20 (2013.01); C07H 21/00 (2013.01); C08G 69/02 (2013.01); C07C 231/02 (2013.01); C07C 233/36 (2013.01); G01N 33/5306 (2013.01); C12Q 1/6876 (2013.01);
Abstract

The invention provides compositions and methods for the detection of targets in a sample; in particular, an in situ hybridization (ISH) sample. Probes and detectable labels may be provided in multiple layers in order to increase the flexibility of a detection system, and to allow for amplification to enhance the signal from a target. The layers may be created by incorporating probes and detectable labels into larger molecular units that interact through nucleic acids base-pairing, including peptide-nucleic acid (PNA) base-pairing. Optional non-natural bases allow for degenerate base pairing schemes. The compositions and methods are also compatible with immunohistochemistry (IHC), immunocytochemistry (ICC), flow cytometry, enzyme immuno-assays (EIA), enzyme linked immuno-assays (ELISA), blotting methods (e.g. Western, Southern, and Northern), labeling inside electrophoresis systems or on surfaces or arrays, and precipitation, among other general detection assay formats. The invention is also compatible with many different types of targets, probes, and detectable labels.

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